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Song sergeant alternative1/5/2024 ![]() Real Friends' contribution to Fearless Records' 2013 Punk Goes Christmas compilation is a desolate yuletide yarn of seasonal solitude. When a song begins with a deathwish on ice, you know you're in for a rueful rumination of past gaffes. Is it better to have loved and lost than never to have loved at all? "You can't miss what you forget." The recently-reunited Mike Kinsella vehicle's spindly guitars, lockstep drums and heartfelt vocals were the soundtrack to a million suburban breakups throughout the 2000s. The starting soliloquy on American Football's influential 1999 debut album, "Never Meant" is a pithy but practical send-off to a squandered relationship. Wait for the final sigh (it's there) and feel free to cry. "Butterfly" wraps up relations, romance and regret into one beautiful, blooming parable of woe. "Yesterday I went outside with my momma's mason jar." Go to a quiet corner, put on your best pair of headphones and play Pinkerton's closing song for a master class in symbolic, sentimental storytelling. "He said: 'I am but one small instrument.'" Spoilers ahead: In a terminal bathroom at the titular airport, the book's main character saves a group of children from a live grenade, dying from blood loss and morbidly fulfilling a lifelong premonition of martyrdom in the process. "We're no saviors if we can't save our brothers," the song's bridge, repeats the sole lyric of Heaven's intro track, "Brothers &"-the tandem suite seemingly referencing the death of a close band associate.Īlso a nod to the band's hometown Phoenix airport, "Goodbye Sky Harbor" recounts the ending of John Irving's 1989 novel, A Prayer For Owen Meany. Pepper's repeating run-out groove at the very end of the cut.Ī serpentine strain of remorse from the Wonder Years' recent No Closer To Heaven, "Cardinals" likens a bird's kamikaze mission to the frittered ashes of blown opportunity and forfeited friendship. As if to add levity to the morbid narrative, frontman Jesse Lacey cheekily apes the Beatles' famous Sgt. The last track on the band's breakthrough Deja Entendu, "Play Crack The Sky" tells the sorrowful tale of a mid-century shipwreck in Montauk, New York, resulting in the loss of 45 lives. Co-written with Cold Cave's Wesley Eisold, Pete Wentz's profoundly forlorn lyrics (vocalized by Patrick Stump's wistful wail) evoke loneliness and failure in a way that few other bands can match. Arguably the most heart-rending Fall Out Boy song yet released, Infinity On High's piano-driven "Golden" can make mincemeat of any melancholy music lover.
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Raindrops keep falling1/5/2024 ![]() Various : The Singer's Anthology of Movie Songs - Men's EditionĪ collection of classic and contemporary songs originally featured in films, in authentic arrangements and appropriate keys. Songlist: Walk On By, Alfie, (There's) Always Something There To Remind Me, The Look Of Love, Anyone Who Had A Heart, The April Fools, (They Long To Be) Close To You, Do You Know The Way To San Jose, Don't Make Me Over, A House Is Not A Home, I Just Don't Know What To Do With Myself, I Say A Little Prayer, I'll Never Fall In Love Again, Magic Moments, Make It Easy On Yourself, Message To Michael, One Less Bell To Answer, Raindrops Keep Fallin' On My Head, This Guy's In Love With You, Trains And Boats And Planes, What The World Needs Now, Wishin' And Hopin', Wives And Lovers (Hey, Little Girl) Voicing: Solo | Performed by Burt Bacharach | 20270b | Songbook | $17.95 | PVG Songlist: Somewhere Out There, That's Entertainment, Born Free, Call Me Irresponsible, Supercalifragilisticexpialidocious, Mona Lisa, Cheek To Cheek, The Exodus Song, Flashdance.What A Feeling, Footloose, Happy Trails, The Heat Is On, I Won't Dance, In The Cool, Cool, Cool Of The Evening, Isn't It Romantic?, Theme From 'Jaws', Just Go To The Movies, The Last Time I Saw Paris, Where Do I Begin (Love Theme), (Meet) The Flintstones, Moon River, Night Fever, Raindrops Keep Fallin' On My Head, Shakedown, Speak Softly, Love (Love Theme), Steppin' Out With My Baby, (Let Me Be Your) Teddy Bear, Thanks For The Memory, Three Coins In The Fountain, Unchained Melody, and more Voicing: SATB | 4340b | Songbook | $3.95īurt Bacharach : The Songs of Bacharach & Hal DavidĢ3 beloved songs by this talented team: Alfie - (There's) Always Something There to Remind Me - Anyone Who Had a Heart - (They Long to Be) Close to You - Do You Know the Way to San Jose - I Say a Little Prayer - I'll Never Fall in Love Again - The Look of Love - Magic Moments - Make It Easy on Yourself - One Less Bell to Answer - Raindrops Keep Fallin' on My Head - Trains and Boats and Planes - Walk On By - What the World Needs Now Is Love - more. Available: SATB Director's Score, SAB Director's Score, 2-Part Director's Score, SATB Singer's Edition, SAB Singer's Edition, 2-Part Singer's Edition, Instrumental Pak, ShowTrax CD. Sections include: The Opening Reel, Thanks for the Memory, Isn't It Romantic?, The Heat Is On, The Envelope Please, Give My Regards to Hollywood. You'll enjoy all the memories, the romance, and the adventure as the cinema comes alive. Spanning eight decades of movie magic, the six flexible sections are easily adaptable to a variety of staging options and can be accompanied with live instruments or the professionally-recorded CD. It does not store any personal data.Mac Huff : Cinemagic! - Hollywood's Best Movie Music (Medley)Ĭelebrate the silver screen and Hollywood's musical history in this choral revue by Mac Huff. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other. The cookies is used to store the user consent for the cookies in the category "Necessary". The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly.
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Baby ninja turtle gif1/4/2024 ![]() ![]() Superhuman Jumping - Leo can jump much higher than a normal human and can leap hundreds of feet into the air and can leap miles in a single jump.Leo might be the fastest of the turtles due to his quick slice and dice fighter style. Superhuman Speed: Despite his size, Leo moves pretty quickly, however this is most likely due to his mutation as well as his training. ![]() Near-Superhuman Strength: Due To his mutation as well as his unique physiology, Leo might be the second strongest turtle, and can deal powerful blows with his feet.Leo has mutated Red Eared Slider turtle Physiology, Giving him athletic abilities beyond even the most dedicated training as well as all of the abilities of a turtle but to a heightened mutated level. Powers and Abilities Mutant Red Eared Slider Physiology Though Leonardo has good traits for a leader he can be irresponsible and immature as seen in Minotaur Maze and Shell in a Cell. Despite his carefree tendencies, Leonardo is capable of being both sensible and serious when the occasion demands it. He could be a strong leader, but he’d rather go for hi-jinx than take the high road. He’s a rebel through and through and isn’t afraid to break the rules when he thinks he’s right. He can easily figure out any situation, and uses his quick-witted skills to beat opponents, clear obstacles, or land one of his many one-liners which he believes "fire up the team." Leo likes to think of himself as the “cool guy” on the team – a rascal with a wink in his eye and a sly grin. Leonardo is charismatic, hilarious, and clever. Leonardo mentioning his winning one liners He also wears a blue belt and strap over his right shoulder, with blue pouches on the belt and a Turtle emblem on the strap. Leo wears a blue face mask over his eyes (with long tails down to his middle back), navy blue fingerless gloves (with blue trim) that extend nearly to his elbows and matching toeless socks that rise to mid-calf. He has black eyes, red markings over his eyes and the sides of his face, yellow marks on his upper arms and thighs, three fingers on each hand and two toes on each foot. New trending GIF tagged dog man hands phone.Leonardo is a lean mutant red-eared turtle with lime green skin, a golden mustard-yellow plastron, and a blue-green carapace with a bright teal design.New trending GIF tagged kpop party hair summer. ![]()
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Connecting to odbc manager in php1/4/2024 Note that if you want to use PHP as a standalone program rather than an Apache module, the tutorial is still relevant. The tutorial describes how to install, configure and test Apache, PHP, the PHP ODBC extension and an Easysoft ODBC Driver. The Easysoft tutorial Enabling ODBC support in PHP under Apache contains installation instructions for the software you need to install before using the PEAR ODBC driver. Installing PHP, Unified ODBC and Easysoft ODBC Drivers To use PEAR DB’s ODBC driver, you need the PHP ODBC extension ( Unified ODBC). PEAR DB layers itself on top of PHP’s existing database extensions.PEAR DB is compatible with PHP 4 (version 4.0.4 or later) and PHP 5 but not PHP 3.However, Easysoft ODBC drivers should work with PHP/PEAR DB on any recent Linux distribution (CentOS, Fedora, Mandrake, SUSE and so on). PHP 5.2.3 and PEAR DB 1.80 on Ubuntu (Edgy Eft, Feisty Fawn, Gutsy Gibbon and Hardy Heron).PEAR DB and LinuxĮasysoft ODBC drivers have been tested with: The PEAR DB structure insulates your PHP applications and scripts from database-specific details letting you switch from one database to another without having to change your PHP code. The default data access methods in common.php are overridden as necessary by the drivers. In the case of PEAR DB’s ODBC driver, the underlying database extension is Unified ODBC.Ĭode shared by the drivers is stored in DB/common.php. The lower level driver files translate between the top-level abstract interface and the database-specific extensions that DB layers itself on top of. The top level, DB.php, provides a database independent interface for interacting with databases. If you want to access your database through ODBC, you need to use PEAR DB rather than PEAR MDB2 therefore. However, it is not currently possible to access a database from PEAR MDB2 through ODBC (see PEAR :: Request #8413 :: ODBC driver). ![]() Note Pear DB has been superceded by Pear MDB2, which is a merge of the DB and Metabase PHP database abstraction layers. You can then access remote database such as Microsoft SQL Server, Oracle®, Microsoft Access, Sybase ASE and InterBase from PEAR DB on Linux and Unix. This tutorial shows how to use PEAR DB with an ODBC driver, which you can download from this site. ![]() The PEAR DB module’s ODBC backend lets you access databases for which an ODBC driver is available. DB provides portability features that enable programs written for one database to work with other databases. One of its most popular packages is PEAR DB, the database abstraction layer created by this project. PEAR (PHP Extension and Application Repository) is a framework and distribution system for reusable PHP classes, libraries and modules. To connect Microsoft Access or any other remote ODBC database to PHP/Pear DB, use PEAR DB with the ODBC-ODBC Bridge. To access Oracle® from PHP/Pear DB, use PEAR DB with the Oracle® ODBC Driver. Use our drivers to connect PHP/PEAR DB to SQL Server 2000-2019 and SQL Server Express. To access Microsoft SQL Server from PHP/PEAR DB running on Unix or Linux, use PEAR DB with the SQL Server ODBC Driver or ODBC-ODBC Bridge (OOB).
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![]() This is also known as Honey or Pulped Natural coffee. This allows for some measure of fermentation to continue throughout the drying process. Medium Fermentation (Semi-washed)įor this method the cherries have their skins removed during the pulping process but instead of completely removing the mucilage, as in the wet process, the sticky flesh layer is left around the beans. This process allows the farmer to carefully control how much fermentation takes place and results in a more consistent coffee with clean and complex flavors. This leaves the beans covered in just a thin sheath, or parchment, called the endocarp. The beans are then washed thoroughly in fresh water to stop the fermentation process and to remove the last of the pulp. The pulped beans are sorted by size and then thrown into fermentation tanks.Īfter 12 to 48 hours of fermentation in the tank, the naturally occurring enzymes dissolve the layer of mucilage surrounding the beans. It has become the most common way of fermenting coffee. This is the more modern, quicker process but it uses a lot of water. Each process has its own logistical pros and cons and the process can have a significant effect on the taste of the final product. There are three main ways of processing the harvested cherries through the fermentation stage. It’s these acids that will be responsible for adding depth and complexity to the coffee. The fermentation process is where the microbial reaction of bacteria and yeasts break down the sugars in the mucilage to produce acids. After depulping, the beans still have some pulp attached and are ready for the fermentation process. This pulp and skin is usually discarded to be used as compost but some “zero waste” coffee producers use these byproducts to make things like tea from the skins ( 4). Within 24 hours of the cherries being picked, they are put through a depulping machine that removes the skin and most of the pulp. A pulper machine is used to mechanically remove the skin of the coffee cherry.
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![]() Taq polymerase preferentially adds a single 3'-A to blunt-ended, double-stranded DNA via this terminal transferase-like activity (13, 14). It has been observed that many DNA polymerases, including Thermus aquaticus (Taq) DNA polymerase, are capable of adding an additional non- template directed nucleotide to the 3'-ends of a blunt-ended DNA fragment. Several methods have now been developed for the cloning of PCR-amplified DNA molecules, including cohesive-end cloning by the introduction of restriction sites at the 5'-ends of PCR primers (7), blunt- end cloning (8, 9), ligation- independent cloning (3-6), and TA cloning (10, 11, 12). Although the development of PCR made the rapid amplification of DNA fragments generally quite simple, the cloning of PCR products was often found to be quite difficult using early technology. Both of the ligase-dependent methods require multiple purification and/or enzymatic modification steps. The other, less efficient approach is blunt-end ligation in which blunt-ended DNA fragments are ligated to a linearized blunt-ended plasmid. However, for every restriction fragment having distinct cohesive ends, one linearized plasmid vector compatible with that (those) particular restriction site(s) has to be prepared. This approach is the most efficient of the two. One approach is cohesive-end ligation, in which cohesive ends on the insert and plasmid are generated by digestion with appropriate restriction enzymes, and the complementary ends of the plasmid and insert are then joined by DNA ligase. There are two subclasses of ligase-dependent methods employed for the covalent joining of a DNA insert to a plasmid vector. This issue was easily resolved conducting an additional Although, the In-Fusion ® cloning method can be used for fusing several fragments in one reaction, in our experience, increasing the number of fragments leads to reduced number of positive clones. The In-Fusion ® enzyme uses a 15-bp overlap between to-be-fused fragments, which can be easily incorporated into primers that are used for PCR amplification. Using this plasmid as a template, we have developed a robust clon- ing method for direct cloning of gRNAs in a plant expres- sion vector using the ligation independent In-Fusion ® cloning system. We developed a unique template plasmid, pEN-Chimera- ccdB carrying the ccdB gene, which virtually eliminated any background plasmids thus greatly accelerating the cloning process for making multiple gRNAs. Taking advantage of this property, multiple cloning methods such as the Gateway ® cloning methods have been developed for reducing background colonies. coli cells that do not carry the antitoxin ccdA gene. The ccdB gene codes for a toxin, which is part of toxin-antitoxin system that inactivates DNA-topoisomerase II complexes thus kill- ing host E. The system developed here utilizes negative selection based on the ccdB gene. This requires screening many colonies to identify positive clones consuming resources and time. Ligation-dependent methods are also prone to getting a larger number of background colonies resulting from the undigested parent vector. Current methods for cloning the target-specific protospacer sequence with an RNA pol III-controlled promoter and the scaffold of sgRNA are time consuming and involve multiple expensive clon- ing steps utilizing type IIS restriction enzymes. The gRNA construct is then usually cloned in a plant expression vector, which carries the Cas9 gene on the same T-DNA cassette. The protospacer for each target has to be cloned between an RNA pol III-controlled promoter and the scaffold of sgRNA. The type II CRISPR/Cas9 system consists of a fixed core RNA-guided DNA nuclease, Cas9, and a chimeric gRNA consisting of a non-variable RNA scaffold derived from the crRNA and tracrRNA and a target-specific 20-nucleotide protospacer RNA, which is engineered specifically for a genomic region to be edited. The CRISPR/Cas9 system has rapidly become a method of choice for genome editing in basic and applied research in various organisms.
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